CD20 Antibody

Catalog Number

250167

Unit

1 ml

Price

$275.00

Format

Each vial contains 1 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Data and Images

Description

This antibody recognizes the CD20 antigen, which is a phosphoprotein with a molecular weight of 35-37 kDa depending of phosphorylation state. CD20 is only expressed on B-lineage cells concomitantly with surface IgM. The CD20 antigen is lost prior to differentiation into plasma cells. No extracellular ligand for CD20 has yet been identified. Indirect evidence suggests that CD20 functions as a B-cell calcium channel subunit. The expression of CD20 in disparate cell types generates a qualitatively similar channel activity to that found endogenously in B-cells. This is consistent with observations that CD20 regulates cell cycle progression and exists on the cell surface as a homo-oligomer. Monoclonal antibodies against CD20 are used to treat people who have B-cells non-Hodgkin's lymphoma (NHL). Various therapeutical forms of these antibodies are known as Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). The Abbiotec antibody can be used for detecting CD20 in flow cytometry.

Product Citations

Mason, D et al. (eds.), Leukocyte typing VII., Oxford University Press, Oxford (2002)

Recommended Products

250166 CD20 Antibody

Alternate Names

<p> B-lymphocyte antigen CD20; Membrane-spanning 4-domains subfamily A member 1; B-lymphocyte surface antigen B1; Leu-16; Bp35; MS4A1; CD21 </p>

Accession No.

P11836
NP_690606

Clonality

CAT 13.6E12

Antigen

Application Notes

FACS with whole blood: 100 μl blood + 10 μl antibody

FACS with cells: 500,000 cells in 50 μl + 10 μl antibody

This antibody recognizes the CD10 antigen. In flow cytometry, the antibody stains 5-10% of human peripheral blood mononuclear cells.

Storage

<p> Store at 4°C. Product is guaranteed 6 months from the date of shipment. </p>