CD45 Antibody

Mouse Monoclonal, PE-conjugated

Catalog Number

250178

Unit

1 ml

Price

$275.00

Format

Each vial contains 1 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Data and Images

CD45 Antibody in Flow Cytometry: The CD45 Antibody (Cat.No.250178) stains human peripheral blood mononuclear cells from lysed whole blood gated on live cells by using flow cytometry.CD45 Antibody in Flow Cytometry: The CD45 Antibody (Cat.No.250178) stains human peripheral blood mononuclear cells from lysed whole blood gated on live cells by using flow cytometry.

Description

This antibody recognizes the CD45 antigen, also named leukocyte common antigen (LCA), since expressed on all leukocytes. The antibody binds an epitope on CD45 named 2b or P. CD45 is a tyrosine phosphatase involved in signal transduction and possibly a regulator of leukocyte activation. The antibody can be used for detecting CD45 in flow cytometry and in immunohistocytology with frozen and paraffin-embedded tissue sections. The epitope 2b on CD45 is resistant to fixation, therefore cells can be pre-fixed before staining with this antibody.



Product Citations

McMichael, AJ et al. (eds), Leukocyte typing III., Oxford University Press, Oxford (1987)

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Alternate Names

Leukocyte common antigen; L-CA; T200; CD45 antigen; PTPRC; Protein tyrosine phosphatase receptor type C

Accession No.

P08575
NP_002830

Clonality

EO1

Antigen

Application Notes

FACS with whole blood: 100 μl blood + 10 μl antibody

FACS with cells: 500,000 cells in 50 μl + 10 μl antibody

This antibody recognizes the 2b epitope (also called P epitope) on CD45 antigen. In flow cytometry, the antibody stains >95% of human peripheral blood mononuclear cells.

 

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.

in

For research use only, not for diagnostic or therapeutic procedures.