DYKDDDDK Tag Antibody

Catalog Number

251329

Unit

0.1 mg

Price

$245.00

Isotype

Applications

Species reactivity

Format

Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.

Description

Epitope tags provide a method to localize gene products in a variety of cell types. The DYKDDDDK tag, also known as Flag tag, is introduced in N-term, C-term and internal position in recombinant proteins. The small size of the epitope tag and its high hydrophilicity does not alter protein expression, proteolytic maturation, antigenicity and function of the fusion protein. An enterokinase cleavage site allows for complete removal of the tag from the fusion protein. Flag is a registered trademark of Sigma-Aldrich.

Recommended Products

350178 DYKDDDDK Peptide

Alternate Names

DYKDDDDK; DYKDDDDK Tag; FLAG; FLAG Tag; FLAG DYKDDDDK; DDK; ECS tag; Enterokinase cleavage site epitope tag; Enterokinase cleavage site

Accession No.

Antigen

KLH-conjugated synthetic peptide DYKDDDDK-C

Application Notes

Cat. No. 251329 binds to both N-term and C-term fusion proteins and does not require Ca2+.

E: 1:500-1:1,000
WB: 1:100-1:500
IHC: 1:100-1:500

Storage

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.