Anti-human IgM Antibody

Secondary, PE-conjugated, Mouse Monoclonal

Catalog Number

250190

Unit

1 ml

Price

$275.00

Isotype

Mouse IgG2a

Applications

E, FACS

Species reactivity

H

Format

Each vial contains 1 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Data and Images

IgM Antibody in Flow Cytometry: The IgM Antibody (Cat.No.250190) stains Daudi cells (fat line) and Ramos cells (thin line) by using flow cytometry.IgM Antibody in Flow Cytometry: The IgM Antibody (Cat.No.250190) stains Daudi cells (fat line) and Ramos cells (thin line) by using flow cytometry.

Description

***DISCONTINUED PRODUCT, REPLACED WITH CAT. NO. 250929*** 

This antibody recognizes human m chains.The specificity of the anti-IgM antibody has been verified in indirect ELISA against human IgM, IgG, IgA and IgD. The antibody can be used for detecting human m heavy chains in ELISA and flow cytometry.


Alternate Names

Immunoglobulin M, type μ heavy chain

Accession No.


Clonality

IIE2

Application Notes

FACS with whole blood: 100 μl blood + 10 μl antibody

FACS with cells: 500,000 cells in 50 μl + 10 μl antibody

This antibody recognizes human μ heavy chains. In flow cytometry, the antibody stains >95% of Ramos cell (λ, μ, δ positive), >95% of Daudi cells (κ, μ, δ positive) and <10% of human peripheral blood mononuclear cells. The antibody does not react with IgG, IgA or IgD.

 

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.

Cellular Antigens,  Immune System

For research use only, not for diagnostic or therapeutic procedures.