AAMP 293 Cell Lysate

Catalog Number

409501

Unit

0.1 mg

Price

$672.00

Applications

Format

This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).

Description

Antigen standard for angio-associated, migratory cell protein (AAMP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.

Application Notes

WB: load 5 ug lysate per lane

Alternate Names

NULL; AAMP;

Accession No.

NP_001078

Storage

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.