Catalog Number

250947

Unit

0.1 mg

Price

$220.00

Isotype

Applications

Species reactivity

Format

Each vial contains 0.1 mg IgM in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by hydroxyapatite chromatography.

Description

The CD14 (HI221) antibody recognizes a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein, which is found on cells of myelomonocytic lineage including monocytes (strongly), granulocytes (weakly) and also on most tissue macrophages. CD14 antigen is also present on reticular dendritic cells, some Langerhans cells, and B lymphocytes (weakly), but is absent from T lymphocytes, NK cells, erythrocytes and platelets. CD14 functions as a high affinity receptor for the complex of lipopolysaccharide (LPS-endotoxin) and LPS-binding protein (LBP), and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble form of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.

Product Citations

Tadamitsu K. et al., eds. 1997. Leucocyte Typing VI: White Cell Differentiation Antigens. P49-52, 113-114  Garland Publishing, Inc., New York.

Alternate Names

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14, urinary form; Monocyte fiferentiation antigen CD14, membrane-bound form; CD14

Accession No.

P08571 [1]
NP_000582 [2]

Clonality

HI221

Application Notes

E: 1:500-1:1,000

FACS: 1:200-1:1,000

Storage

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.