Catalog Number

250162

Unit

0.1 mg

Price

$515.00

Isotype

Applications

Species reactivity

Format

Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Description

***DISCONTINUED PRODUCT***

This antibody recognizes the CD14 antigen, a LPS receptor which is strongly expressed on monocyte surface and weakly expressed on granulocyte, macrophage, dendritic cell and B-cell surface.  This antibody neutralizes LPS-mediated cell activation. The antibody can be used for detecting CD14 in flow cytometry and in immunohistocytology with frozen and paraffin-embedded tissue sections.

Product Citations

Mason, D et al. (eds.), Leukocyte typing VII., Oxford University Press, Oxford (2002)

Recommended Products

250160 CD14 Antibody [1]
250159 CD14 Antibody [2]
250161 CD14 Antibody [3]

Alternate Names

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14, urinary form; Monocyte fiferentiation antigen CD14, membrane-bound form; CD14

Accession No.

P08571 [4]
NP_000585 [5]

Clonality

18D11

Application Notes

FACS with whole blood: 100 μl blood + 10 μl antibody

FACS with cells: 500,000 cells in 50 μl + 10 μl antibody

This antibody recognizes the CD14 antigen. In flow cytometry, the antibody stains more than 90% of human peripheral blood monocytes. The antibody is LPS neutralizing.

Storage

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.