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Protein Kinases

ROR1 293 Cell Lysate

$392.00
SKU: 409498

Antigen standard for receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC224765, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.

List price: $0.00
Image 
ROR1 lysate validation: The ROR1 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-ROR1 with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
Enzymes
Protein Kinases
Receptors and Channels
Pathway and Disease 
Nervous and Sensory Systems
Signaling Molecules and Interaction
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
IP
WB
Alternate Names 
isoform 2 is encoded by transcript variant 2; neurotrophic tyrosine kinase receptor-related 1; neurotrophic tyrosine kinase, receptor-related 1; tyrosine-protein kinase transmembrane receptor ROR1; ROR1; dJ537F10.1; MGC99659; NTRKR1
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_001077061

MAP2K2 293 Cell Lysate

$392.00
SKU: 402673
Antigen standard for mitogen-activated protein kinase kinase 2 (MAP2K2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201827, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
MAP2K2 lysate validation: The MAP2K2 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-MAP2K2 with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
Enzymes
Protein Kinases
Pathway and Disease 
Cancers
Signal Transduction
Immune System
Cell Communication
Cell Motility
Nervous and Sensory Systems
Endocrine System
ORF Clone URL 
http://www.origene.com/orf_clone/search/retrieve_results.mspx?accn=NM_030662&sku...
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
mitogen-activated protein kinase kinase 2, p45; MAP kinase kinase 2; MAPK/ERK kinase 2; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MEK 2; MAPKK 2; MAP2K2; FLJ26075; MAPKK2; MEK2; MKK2; PRKMK2
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_109587

ACVR2B 293 Cell Lysate

$672.00
SKU: 400459
Antigen standard for activin A receptor, type IIB (ACVR2B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC212816, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
ACVR2B lysate validation: The ACVR2B 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-ACVR2B with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $672.00
Protein Family 
Enzymes
Cytokines
Receptors and Channels
Protein Kinases
Pathway and Disease 
Signaling Molecules and Interaction
Signal Transduction
ORF Clone URL 
http://www.origene.com/orf_clone/trueclone/accession/NM_001106/RC212816.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
activin receptor type-2B; ACVR2B; ActR-IIB; ACTRIIB; MGC116908
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_001097

MET 293 Cell Lysate

$672.00
SKU: 400183
Antigen standard for met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC217003, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
MET lysate validation: The MET 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-MET with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $672.00
Protein Family 
Enzymes
Protein Kinases
Receptors and Channels
Pathway and Disease 
Cancers
Cell Communication
Development and Behavior
Infectious Diseases
Signaling Molecules and Interaction
ORF Clone URL 
http://www.origene.com/orf_clone/trueclone/NM_000245/RC217003/MET.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
oncogene MET; HGF receptor; scatter factor receptor; SF receptor; met proto-oncogene tyrosine kinase; HGF/SF receptor; proto-oncogene c-Met; tyrosine-protein kinase Met; hepatocyte growth factor re; MET; AUTS9; c-Met; HGFR; RCCP2
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_000236

Human VEGFR-1

$235.00
SKU: 600311

Vascular Endothelial Growth Factor Receptor-1 (VEGFR-1) belongs to a family of five receptor tyrosine kinase receptors that are specific to a number of angiogenic factors including VEGF and PDGF, and are therefore thought to play a role in the angiogenic process. VEGFR-1 is expressed in two forms, predominantly on endothelial cells, giving rise to a full-length membrane bound receptor and a truncated soluble receptor. Elevated levels of the soluble form of this VEGF receptor are used in the diagnosis of pathological conditions.

List price: $0.00
Price: $235.00

Human ILK-1

$235.00
SKU: 600258

Integrin-linked kinase (ILK-1) acts as a mediator of inside-out integrin signaling. It is part of focal adhesion complex ILK-PINCH, considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Integrin-linked kinase is implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Integrin-linked kinase phosphorylates beta-1 and beta-3 integrin subunits on serine and threonine residues.

List price: $0.00
Price: $235.00

Human CDK4

$235.00
SKU: 600236
Cyclin-Dependent Kinase 4 (CDK4) is involved in cell cycle control. Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Recombinant human CDK4 is expressed in E. coli with an amino-terminal hexahistidine tag
List price: $0.00
Price: $235.00

VEGFR2 Antibody

$315.00
SKU: 254506
VEGFR-2 (Vascular endothelial growth factor receptor 2) is a tyrosine-protein kinase and receptor for VEGF or VEGFC. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In HIV-1 infection cases, VEGFR2 interaction with extracellular viral Tat protein enhances angiogenesis in Kaposi's sarcoma lesions.
List price: $0.00
Price: $315.00

IRAK (8F1A7) Antibody

$310.00
SKU: 253285
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently.
List price: $0.00
Price: $310.00

IRAK (1F12C1) Antibody

$310.00
SKU: 253284
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently.
List price: $0.00
Price: $310.00

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