Antigen standard for receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC224765, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Vascular Endothelial Growth Factor Receptor-1 (VEGFR-1) belongs to a family of five receptor tyrosine kinase receptors that are specific to a number of angiogenic factors including VEGF and PDGF, and are therefore thought to play a role in the angiogenic process. VEGFR-1 is expressed in two forms, predominantly on endothelial cells, giving rise to a full-length membrane bound receptor and a truncated soluble receptor. Elevated levels of the soluble form of this VEGF receptor are used in the diagnosis of pathological conditions.
Integrin-linked kinase (ILK-1) acts as a mediator of inside-out integrin signaling. It is part of focal adhesion complex ILK-PINCH, considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Integrin-linked kinase is implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Integrin-linked kinase phosphorylates beta-1 and beta-3 integrin subunits on serine and threonine residues.