Human aFGF

Catalog Number

600111

Unit

0.05 mg

Price

$235.00

Applications

Format

Each vial contains 50 ug protein lyophilized in 75 mM NaCl, 10 mM sodium phosphate pH7.5. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Description

Acidic fibroblast growth factor (aFGF or Heparin-Binding growth factor 1) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. aFGF binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. Recombinant Human Acidic Fibroblast Growth Factor produced in E. coli is a non-glycosylated single polypeptide chain containing 141 amino acids with a MW of 15,967 Da.

Alternate Names

Heparin-Binding growth factor 1; HBGF-1; acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; ECGF-beta; FGF1; FGFA

Accession No.

P05230

MW

15967 Da

Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Human Fibroblast Growth Factor-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1.0 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.