Human MIP-1-Alpha

Catalog Number

600154

Unit

0.02 mg

Price

$235.00

Applications

Format

Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Description

Recombinant Human MIP-1 produced in E.Coli is a single non-glycosylated polypeptide chain containing 70 amino acids with a MW of 7,820 Da.

Alternate Names

C-C motif chemokine 3; small-inducible cytokine A3; macrophage inflammatory protein 1-alpha; MIP-1-alpha; tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; G0S19-1 protein; SIS- beta; PAT 464.1; MIP-1-alpha(4-69); LD78-alpha(4-69); CCL3; G0S19-1; MIP1A; SCYA3

Accession No.

P10147

MW

7820 Da

Sequence

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Determined by its ability to chemoattract human monocytes using a concentration range of 0.5 - 8 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.