Human SDF-1-alpha

Catalog Number

600171

Unit

0.01 mg

Price

$235.00

Applications

Format

Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Description

Recombinant Human SDF-1 produced in E. coli is a non-glycosylated polypeptide chain containing 68 amino acids with a MW of 8,008 Da.

Alternate Names

Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Pre-B cell growth stimulating factor; PBSF; Intercrine reduced in hepatomas; IRH; hIRH; SDF-1-beta(3-72); SDF-1-alpha(3-67); CXCL12; SDF1; SDF1A; SDF1B

Accession No.

P48061

MW

8008 Da

Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

SDF-1a is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 10 - 75 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.