Custom Antibody Production

Abbiotec offers custom polyclonal antibody production services to the scientific community to accelerate a project From Biology to Discovery™. When the antibody for a chosen antigen is not already available off the shelf, it is time to develop a custom project. The experienced staff of Abbiotec processes a custom project with the same mindset and tools that it develops antibody products for the Abbiotec catalog: we want an antibody that specifically detects the antigen in the chosen application.

Polyclonal Antibody Production Services

Abbiotec has simplified the ordering process by offering 3 packages combining the most common customer requests:

Protein-specific Ab Combo: With 3 mg of protein as antigen, 50-100 mg of purified antibody is generated in rabbits against multiple epitopes within 3 months.

Peptide-specific Ab Combo: A peptide encompassing a short region of the target protein is used as antigen to generate in rabbits 50-100 mg of purified antibody within 4 months.

Phospho-specific Ab Combo: For monitoring target activation by phosphorylation, a phosphopeptide is used as antigen to generate in rabbits 5-10 mg of affinity-purified antibody within 4 months.

Abbiotec offers complimentary peptide design services to ensure the success of the project.

Cat. No.	100100	100200	100300
Polyclonal Antibody Combo	Protein-specific Ab	Peptide-specific Ab	Phospho-specific Ab
Immunogen	Protein	Peptide	phospho-peptide
Peptide synthesis, purity>70%		√
Peptide synthesis, purity>90%		Optional	√
Peptide-KLH conjugation		√	√
Immunization and sera collection	√	√	√
Titration by ELISA	√	√	√
Protein-G purification	√	√
Antigen-affinity purification	Optional	Optional	√
Time	3 months	4 months	4 months
Price	$1,000.00	$1,500.00	$3,000.00

Abbiotec offers numerous other antibody production services to customize a project to the investigator’s request. A non-exhaustive list is displayed below. For more information, please contact Technical Service Dept.

Cat. No.	Polyclonal Antibody Services	Price
100100	Protein-specific Ab Combo Cat No. 100120, 100130, 100150	$1,000.00
100200	Peptide-specific Ab Combo Cat No. 100210, 100110, 100120, 100130, 100150	$1,500.00
100300	Phospho-specific Ab Combo Cat No. 100220, 100110, 100120, 100130, 100320	$3,000.00
100210	Peptide synthesis, purity>70%, 15-19 mg	$300.00
100220	Peptide synthesis, purity>90%, 15-19 mg	$500.00
100310	Phospho-peptide synthesis, purity>90%, 15-19 mg	$800.00
100110	Peptide-carrier bioconjugation	$200.00
100120	Animal immunization and antisera production (2 rabbits)	$600.00
100130	Titer test by ELISA	$100.00
100140	SAS purification	$200.00
100150	Protein-G purification	$300.00
100160	Antigen-affinity purification	$500.00
100320	2-step phospho-specific purification by peptide affinity	$1,000.00

Get a Quotation

Since prices vary upon project customization, we recommend submitting a quotation using the Quote Form for obtaining an accurate price. All requests are processed within 48 hrs.

Guarantees

Abbiotec guarantees competitive pricing and delivery time for all routine options.

Every antibody project is supplied with a Peptide Design and Project reports, when applicable. Abbiotec guarantees that the antibody generated using an antigen synthesized by Abbiotec will recognize the antigen in ELISA.

Althought most projects can be completed within 3-4 months, actual processing time is dependent on the peptide synthesis and animal immunization processes. Please contact the Technical Service Dept for updated timelines. Estimated delivery times are stated on all quotations.