Anti-human IgG (4D2D) Antibody
Monoclonal anti-human IgG is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no cross-reactivity with human IgM molecule as determined by ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG. The antibody binding site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.